pegfp vamp4 Search Results


vamp4  (Addgene inc)
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Addgene inc vamp4
FIGURE 2 | VAMP7 shows a high degree of co-localization with cytotoxic granules. (A) SIM images of bead-stimulated human CD8+ T cells co-transfected with either granzyme B-mCherry or granzyme B-mTFP along with the v-SNAREs mVAMP2, VAMP3, <t>VAMP4,</t> VAMP7, and VAMP8 constructs. Overlay of both channels displayed that only VAMP7 co-localizes with the lytic granule marker granzyme B. (B) Pearson’s and (C) Manders’ overlap coefficients for co-localization of VAMP3 (Continued)
Vamp4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIGURE 2 | VAMP7 shows a high degree of co-localization with cytotoxic granules. (A) SIM images of bead-stimulated human CD8+ T cells co-transfected with either granzyme B-mCherry or granzyme B-mTFP along with the v-SNAREs mVAMP2, VAMP3, VAMP4, VAMP7, and VAMP8 constructs. Overlay of both channels displayed that only VAMP7 co-localizes with the lytic granule marker granzyme B. (B) Pearson’s and (C) Manders’ overlap coefficients for co-localization of VAMP3 (Continued)

Journal: Frontiers in immunology

Article Title: Cytotoxic Granule Exocytosis From Human Cytotoxic T Lymphocytes Is Mediated by VAMP7.

doi: 10.3389/fimmu.2019.01855

Figure Lengend Snippet: FIGURE 2 | VAMP7 shows a high degree of co-localization with cytotoxic granules. (A) SIM images of bead-stimulated human CD8+ T cells co-transfected with either granzyme B-mCherry or granzyme B-mTFP along with the v-SNAREs mVAMP2, VAMP3, VAMP4, VAMP7, and VAMP8 constructs. Overlay of both channels displayed that only VAMP7 co-localizes with the lytic granule marker granzyme B. (B) Pearson’s and (C) Manders’ overlap coefficients for co-localization of VAMP3 (Continued)

Article Snippet: VAMP3, VAMP4, VAMP7, and VAMP8 constructs were purchased from Addgene (42310, 42313, 42316, and 42311).

Techniques: Transfection, Construct, Marker

FIGURE 3 | VAMP7 polarizes to the IS on the same granules as granzyme B and perforin. (A) SIM images of SEA-pulsed human CD8+ T cells transfected with VAMP7-pHuji construct and conjugated with SEA pulsed RAJI cells and fixed after 5, 10, and 15 min, respectively. CTLs were permeabilized and immunolabeled with either Alexa647-conjugated granzyme B or Alexa647-conjugated perforin antibody. (B,C) The corresponding Pearson’s overlap coefficients for co-localization of VAMP7-mCherry with granzyme B (n = 12) and VAMP7-pHuji with perforin (n = 11) are given in the text. Data are shown as mean ± SEM. Scale bar, 5 µm. (D) SIM images of SEA-pulsed human CD8+ T cells transfected with VAMP2-pHuji, EGFP-VAMP3, and EGFP-VAMP4 constructs and conjugated with SEA pulsed RAJI cells and fixed after 15 min. CTLs were permeabilized and immunolabeled with either Alexa647-conjugated granzyme B or Alexa647-conjugated perforin antibody. (E,F) The corresponding Pearson’s overlap coefficients for co-localization of VAMP2, VAMP3, and VAMP4 with granzyme B (n = 15) or perforin (n = 16) are given in the text. Data are shown as mean ± SEM. Scale bar, 5 µm.

Journal: Frontiers in immunology

Article Title: Cytotoxic Granule Exocytosis From Human Cytotoxic T Lymphocytes Is Mediated by VAMP7.

doi: 10.3389/fimmu.2019.01855

Figure Lengend Snippet: FIGURE 3 | VAMP7 polarizes to the IS on the same granules as granzyme B and perforin. (A) SIM images of SEA-pulsed human CD8+ T cells transfected with VAMP7-pHuji construct and conjugated with SEA pulsed RAJI cells and fixed after 5, 10, and 15 min, respectively. CTLs were permeabilized and immunolabeled with either Alexa647-conjugated granzyme B or Alexa647-conjugated perforin antibody. (B,C) The corresponding Pearson’s overlap coefficients for co-localization of VAMP7-mCherry with granzyme B (n = 12) and VAMP7-pHuji with perforin (n = 11) are given in the text. Data are shown as mean ± SEM. Scale bar, 5 µm. (D) SIM images of SEA-pulsed human CD8+ T cells transfected with VAMP2-pHuji, EGFP-VAMP3, and EGFP-VAMP4 constructs and conjugated with SEA pulsed RAJI cells and fixed after 15 min. CTLs were permeabilized and immunolabeled with either Alexa647-conjugated granzyme B or Alexa647-conjugated perforin antibody. (E,F) The corresponding Pearson’s overlap coefficients for co-localization of VAMP2, VAMP3, and VAMP4 with granzyme B (n = 15) or perforin (n = 16) are given in the text. Data are shown as mean ± SEM. Scale bar, 5 µm.

Article Snippet: VAMP3, VAMP4, VAMP7, and VAMP8 constructs were purchased from Addgene (42310, 42313, 42316, and 42311).

Techniques: Transfection, Construct, Immunolabeling